SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expressionArticle Published on 2022-11-012022-11-16 Journal: Life sciences [Category] SARS, 유전자 메커니즘, [키워드] A549 ACE2 ACE2 expression angiotensin ATP synthase autophagy BEAS-2B Brain Caco-2 can be used Cell cell line cell lines cellular cellular metabolism COVID-19 COX endothelial energy metabolism evaluated expression level HEK-293 human cell human cell line in vitro intestine intrinsically investigated kidney liver mechanism metabolism Mitochondria mitochondrial metabolism modified cell line molecular mechanism mRNA expression NDUFS2 overexpressed overexpressing overexpression Protein Replication replication kinetics respiratory tract RT-qPCR SARS-COV-2 infection SARS-CoV-2 replication SH-SY5Y susceptibility susceptible cell TMPRSS2 TMPRSS2 expression TMPRSS2. uninfected cell various tissue viral infection Viral load viral replication were assessed western blot [DOI] 10.1016/j.lfs.2022.120930 PMC 바로가기
SARS-CoV-2 modulates virus receptor expression in placenta and can induce trophoblast fusion, inflammation and endothelial permeabilityArticle Published on 2022-09-132022-11-16 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, [키워드] ACE2 addition Apoptosis CD147 Cell cell lines cells Coagulation complement Cytokine storm Effect elevated elicit endothelial event Evidence expression fetal Hyperactivation immunopathological Impact incidence increased vascular permeability induce infected with SARS-CoV-2 Infection Inflammation less main receptor mechanism modulate Mortality mother outcomes pathway Placenta placental positive Pregnancy pregnancy. pregnant Preterm birth pro-inflammatory cytokine Protein raise SARS-CoV-2 spike susceptibility syndrome the SARS-CoV-2 TMPRSS2 TMPRSS2 expression Transmission upregulated virus virus receptor [DOI] 10.3389/fimmu.2022.957224 PMC 바로가기
Acquisition of Furin Cleavage Site and Further SARS-CoV-2 Evolution Change the Mechanisms of Viral Entry, Infection Spread, and Cell Signaling푸린 절단 부위의 획득 및 추가 SARS-CoV-2 진화는 바이러스 진입, 감염 확산 및 세포 신호 전달의 메커니즘을 변경합니다Article Published on 2022-08-102022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 acquisition activate activating Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Adaptive immune response alphavirus appear cathepsin Cathepsins Cell Cell fusion change coronavirus COVID COVID-19 disease Cytokines Deletion determined by endosome Entry mechanism entry pathway Evolution experimental system expressing S protein expression FCS furin furin cleavage furin cleavage site hallmark human population immune in vitro induce infected with SARS-CoV-2 infecting Infection inhibitor inhibitors lack leads Lineage mAbs mechanism media mRNA vaccines Neutralizing neutralizing monoclonal antibodies neutralizing monoclonal antibody pro-inflammatory cytokines pro-inflammatory response pseudoinfectious viruses pseudovirus pseudoviruses receptor released respiratory S protein S1 secretion S1 subunit SARS-CoV-2 SARS-CoV-2 pathogenesis SARS-CoV-2 S protein secrete sera severe acute respiratory syndrome Coronavirus shown Site Spike protein Spread spreading Syncytia syncytium syncytium formation the S protein the S1 subunit TLR4 TMPRSS2 TMPRSS2 expression variant Viral viral evolution viral evolution. virus virus entry virus spread [DOI] 10.1128/jvi.00753-22 PMC 바로가기 [Article Type] Article
Functional properties of the spike glycoprotein of the emerging SARS-CoV-2 variant B.1.1.529신흥 SARS-CoV-2 변이체 B.1.1.529의 스파이크 당단백질의 기능적 특성Article Published on 2022-06-142022-09-11 Journal: Cell Reports [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 affinity ACE2 binding acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 B.1.1.529 B.1.617.2 Cell coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CP: Immunology CP: Microbiology cytopathic effects D614G decrease exhibit functional glycoprotein higher affinity immune evasion immunology Infection less Microbiology neutralization neutralization sensitivity omicron Patient prevalent processing property pseudovirus pseudoviruses relative S glycoprotein S glycoproteins S trimer S1 shedding SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sera severe acute respiratory syndrome Coronavirus spike glycoprotein stability syncytium formation temperature TMPRSS2 TMPRSS2 expression Transmission unliganded vaccinees variant variants variants. virion [DOI] 10.1016/j.celrep.2022.110924 PMC 바로가기 [Article Type] Article
Periodontitis promotes the expression of gingival transmembrane serine protease 2 (TMPRSS2), a priming protease for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)치주염은 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 프라이밍 프로테아제인 치은 막횡단 세린 프로테아제 2(TMPRSS2)의 발현을 촉진합니다Article Published on 2022-06-012022-09-11 Journal: Journal of oral biosciences [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome Analysis analyzed anti-TMPRSS2 antibody cell membrane ChIP coronavirus correlation COVID-19 dataset datasets ESR1 Estrogen estrogen receptor Express expressed expression Gene Expression Gene Expression Omnibus GEO GEO dataset Gingiva Human increase increased expression investigated keratinocytes Ligand oral cavity Periodontitis priming promote promoter promoter region protease Quantitative responsible reverse transcription SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2. screened Serine Spike protein the SARS-CoV-2 tissue tissues TMPRSS2 TMPRSS2 expression transcription factor transcription factors transmembrane serine protease transmembrane serine protease 2 was performed western blot Western blot analysis [DOI] 10.1016/j.job.2022.04.004 PMC 바로가기 [Article Type] Article
SMYD2 Inhibition Downregulates TMPRSS2 and Decreases SARS-CoV-2 Infection in Human Intestinal and Airway Epithelial CellsSMYD2 억제는 인간 장 및 기도 상피 세포에서 TMPRSS2를 하향 조절하고 SARS-CoV-2 감염을 감소시킵니다Article Published on 2022-04-082022-09-11 Journal: Cells [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, 치료제, [키워드] antiviral activity Antiviral treatment antiviral treatment. AZ505 BAY598 block caused cellular circulating COVID-19 COVID-19 pandemic cultured cell cultured cells curtail decrease deficiency delta variant detectable downregulate effective antiviral treatment Effective vaccines exhibit expresse had no Human infect inhibited inhibition inhibitor lysine mRNA Neutralizing antibodies neutralizing antibody no effect not express pharmacological priming protein levels reporter SARS-CoV-2 SARS-COV-2 infection significantly SMYD2 Spike protein target cells targeting the spike protein TMPRSS2 TMPRSS2 expression Treatment variant Vero E6 VERO E6 cells Viral viral replication virus virus replication virus variant virus variants [DOI] 10.3390/cells11081262 PMC 바로가기 [Article Type] Article
New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts Research Published on 2022-03-112022-10-05 Journal: Cell Death Discovery [Category] 진단, 치료기술, 치료법, [키워드] ACE2 AKT cannabis caused Cell Combination components COVID-19 COX2 downregulated Epigenetic expression Extract Factor Features fibroblast highlighting IL-6 IL-8 inflammatory mediator inhibited Interaction lung mechanism miRNA modulated New pathway proinflammatory suppressed target cell the SARS-CoV-2 virus TMPRSS2 TMPRSS2 expression [DOI] 10.1038/s41420-022-00876-y [Article Type] Research
Well-differentiated liver cancers reveal the potential link between ACE2 dysfunction and metabolic breakdown잘 분화된 간암은 ACE2 기능 장애와 대사 장애 사이의 잠재적 연관성을 보여줍니다Article Published on 2022-02-032022-09-10 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] ACE2 acute respiratory syndrome coronavirus Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 bile canaliculi bioinformatics bystander Cancer Cancer cells cancers Cell co-expression network coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 dataset DNA dysfunction endothelial cells enzyme Epigenetics analysis Express FIVE followed by functions Gene expression analysis Genomic analysis hepatic function hepatocytes High-resolution human liver Image analysis imaging immunohistochemistry Immunological techniques in vitro Inflammation liver Liver cancer Liver disease livers Localization Microarray Microscopy mRNA multiplex mutated oxidative stress POLE predicted promoter protein expression Quantitative receptor respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infection in vitro Sequencing Serine serine protease severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 tissue TMPRSS2 TMPRSS2 expression transcriptomic transmembrane serine protease transmembrane serine protease 2 Tumor tumors Viral whole mount β-catenin [DOI] 10.1038/s41598-021-03710-0 PMC 바로가기 [Article Type] Article
Effects of acute severe acute respiratory syndrome coronavirus 2 infection on male hormone profile, ACE2 and TMPRSS2 expression, and potential for transmission of severe acute respiratory syndrome coronavirus 2 in semen of Asian men급성 중증 급성 호흡기 증후군 코로나바이러스 2 감염이 남성 호르몬 프로필, ACE2 및 TMPRSS2 발현에 미치는 영향, 아시아 남성의 정액에서 중증 급성 호흡기 증후군 코로나바이러스 2 전파 가능성Article Published on 2022-02-012022-09-11 Journal: F&S science [Category] COVID19(2023년), SARS, 치료기술, [키워드] ACE2 ACE2 and TMPRSS2 expression ACE2 expression Acute COVID-19 infection acute phase acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 acute SARS-CoV-2 infection affected age androgen Asian binding changes in clinical clinical study Cohort Contact coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 infection detect detectable diagnosis of SARS-CoV-2 diagnostic Effect expressed expression expression of SARS-CoV-2 follicle-stimulating hormone Human Infection Laboratory laboratory-confirmed Luteinizing Hormone male male hormone male hormone profile masturbation men Mild molecular morning Nasopharyngeal swab normal range outcome potential for transmission profile recruited RNA SARS-CoV-2 SARS-CoV-2 expression SARS-COV-2 infection SARS-CoV-2 RNA Semen semen. severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Sex sex hormone Singapore Symptom Testosterone TMPRSS2 TMPRSS2 expression undetectable university was collected [DOI] 10.1016/j.xfss.2021.11.003 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Omicron variant shows less efficient replication and fusion activity when compared with Delta variant in TMPRSS2-expressed cellsSARS-CoV-2 Omicron 변이체는 TMPRSS2 발현 세포에서 Delta 변이체와 비교할 때 복제 및 융합 활성이 덜 효율적입니다.Comparative Study Published on 2022-01-192022-09-10 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, [키워드] Affect Antiviral effect B.1.1.529 bafilomycin Calu3 CaLu3 cells camostat Cell cell line cells Characteristics Chloroquine clinical manifestation clinical manifestations competition Delta delta variant Delta variants disease severity Endocytic pathway entry pathway Immune escape Infection inhibit inhibited inhibiting less mutations not enhanced omicron Omicron variant replicated Replication robust SARS-CoV-2 Serine TMPRSS2 TMPRSS2 expression TMPRSS2 pathway transmembrane serine protease transmembrane serine protease 2 Transmissibility variant variants viral replication Virological [DOI] 10.1080/22221751.2021.2023329 PMC 바로가기 [Article Type] Comparative Study